Prof. Frank DUBOIS, ULB
Frank Dubois: born in October, 1956, in Brussels, he received his Master’s degree in physics in 1979 from the Université Libre de Bruxelles, Belgium. He became a physics teacher in College for a few years before returning to Research. He received his Ph.D. in Physics in 1992 from the same University. His Ph.D. thesis was focused on pattern recognition by correlation methods. He joined the Microgravity Research Centre in 1996 where he was in charge of the optical system for space application. In 2002 he got tenure and became Professor in 2003. In October 2007 he was nominated head of the MRC. He started his activity on December 30th 2002, in the Optoelectronic Division. Since 1999 his activity is focused on digital holography microscopy. He is co-author of tens of articles published in several high level international journals as well as many communications at international conferences. He participated in several conferences as invited speaker. He applied for 3 patents 2 of which became US patents. He is also co-author of 3 chapters in international books. He acts as a referee for several high level international journals, such as Optical Express, Applied Optics, Optics Letters and Measurement Science and Technology.
Dr Annick BRANDENBURGER, Ph. D.
Annick BRANDENBURGER was born in 1954 in Luxemburg. She studied at the “Université Libre de Bruxelles (ULB), Belgium and graduated in 1977 with a master’s degree in molecular biology. She presented a PhD thesis on “Genetic and biochemical studies of inducible repair functions in Escherichia coli” at the same University in 1982. Part of her PhD studies were performed in Leiden (Netherlands), in the laboratorium voor moleculaire genetica of prof Glickman (1979-1980) and in Gif-sur-Yvette (France) in the laboratoire d'enzymologie, (CNRS) of prof Devoret (1980-1982). She continued her career at the ULB where she supervised the master’s and PhD studies of several students. Her work also involved teaching: virology and oncology (1992-1995), and lecturing: vectors for gene therapy (1997-2009). She was member of a group of experts for the Belgian Biosafety Advisory Council (expert group: recombinant viral vectors, virosomes, vaccines, gene therapy) (-2009). Her publications comprise papers in international journals (20 publications), monographies (5) and reports of international meetings (21). Research interests: microbiology, bacterial genetics, mutagenesis, DNA repair (1977-1985), parvoviruses, cancer immuno-therapy (1986-2007), generation of tumour cell-dendritic cell hybrids for anti-tumour vaccination (2003- ).
- F. Dubois, C. Yourassowsky, Patent n° US 7,463,366 B2, December 9 (2008)
- F. Dubois, C. Yourassowsky, Patent n° US 7362449 B2, April 22 (2008)
- F. Dubois, C. Yourassowsky, EP 1 631 788 B1, March 14 (2007)
- F. Dubois, C. Yourassowsky, Patent n° US 7009700 B2, March 7 (2006)
- F. Dubois, et al., Appl. Opt. 38, 7085-7094 (1999)
- F. Dubois et al., Jour. of Biomed. Opt. 11-5, 054032 (2006)
Description and applications
Digital holographic microscopy allows to record the information of an entire experimental volume as a hologram. The hologram can be treated to refocus the sample at different depths without temporal distortion. It also allows to carry out the interferometric measure of optical thickness with nanometric precision (quantitative phase contrast)
This optical microscopy technique has applications in numerous research fi elds such as monitoring of cells in culture, precise measurements inside cells (i.e. refractive index), the observation of particles in a fl ux and 3D tomography.
The laboratory of Physical Chemistry of the Université Libre de Bruxelles (ULB) has developed an original holographic microscopy technology. That is covered by a number of patents. It generates images with a quality similar to that of classical microscopes in different modes such as bright fi eld, DIC and quantitative phase contrast with a high capacity of in depth investigation, as well as the combination with fl uorescence.
The digital holographic microscopy has been applical to many areas of research monitoring of cell cultures, cell fusion, migration of tumour cells in thick collagen gels as well as velocimetry and the analysis of particles in a fl uid. A patented microscope has also been used in the International Space Station (ISS) for the observation of protein crystallization in microgravity.
See the poster: POSTER_CMMI_-_Holographic_Microscopy.pdf